Chitinase genes LbCHI31 and LbCHI32 from Limonium bicolor were successfully expressed in Escherichia coli and exhibit recombinant chitinase activities.

ABSTRACT: The two chitinase genes, LbCHI31 and LbCHI32 from Limonium bicolor, were, respectively, expressed in Escherichia coli BL21 strain. The intracellular recombinant chitinases, inrCHI31 and inrCHI32, and the extracellular exrCHI31 and exrCHI32 could be produced into E. coli. The exrCHI31 and exrCHI32 can be secreted into extracellular medium. The optimal reaction condition for inrCHI31 was 5 mmol/L of Mn²⁺ at 40°C and pH 5.0 with an activity of 0.772 U using Alternaria alternata cell wall as substrate. The optimal condition of inrCHI32 was 5 mmol/L of Ba²⁺ at 45°C and pH 5.0 with an activity of 0.792 U using Valsa sordida cell wall as substrate. The optimal reaction condition of exrCHI31 was 5 mmol/L of Zn²⁺ at 40°C and pH 5.0, and the activity was 0.921 U using the A. alternata cell wall as s

SUBMITTER: Liu Z 

PROVIDER: S-EPMC3872228 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature