HLA class I alleles are associated with peptide-binding repertoires of different size, affinity, and immunogenicity.

ABSTRACT: Prediction of HLA binding affinity is widely used to identify candidate T cell epitopes, and an affinity of 500 nM is routinely used as a threshold for peptide selection. However, the fraction (percentage) of peptides predicted to bind with affinities of 500 nM varies by allele. For example, of a large collection of ~30,000 dengue virus-derived peptides only 0.3% were predicted to bind HLA A*0101, whereas nearly 5% were predicted for A*0201. This striking difference could not be ascribed to variation in accuracy of the algorithms used, as predicted values closely correlated with affinity measured in vitro with purified HLA molecules. These data raised the question whether different alleles would also vary in terms of epitope repertoire size, defined as the number of associated epitopes or,

SUBMITTER: Paul S 

PROVIDER: S-EPMC3872965 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature