Detection of serotype-specific antibodies to the four dengue viruses using an immune complex binding (ICB) ELISA.

ABSTRACT:

Background

Dengue virus (DENV) infections are preferentially diagnosed by detection of specific IgM antibodies, DENV NS1 antigen assays or by amplification of viral RNA in serum samples of the patients. The type-specific immunity to the four worldwide circulating DENV serotypes can be determined by neutralization assays. An alternative to the complicated neutralization assays would be helpful to study the serotype-specific immune response in people in DENV hyperendemic areas but also in subjects upon DENV vaccination.

Methods

In consecutive samples of patients with DENV-1- 4 infection type-specific antibodies were detected using an immune complex binding (ICB) ELISA. During incubation of serum samples and enzyme- labeled recombinant envelope domain III (EDIII) antigens immun

SUBMITTER: Emmerich P 

PROVIDER: S-EPMC3873247 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature