Functional and evolutionary genomics of Mycobacterium tuberculosis: insights from genomic deletions in 100 strains.

ABSTRACT: To better understand genome function and evolution in Mycobacterium tuberculosis, the genomes of 100 epidemiologically well characterized clinical isolates were interrogated by DNA microarrays and sequencing. We identified 68 different large-sequence polymorphisms (comprising 186,137 bp, or 4.2% of the genome) that are present in H37Rv, but absent from one or more clinical isolates. A total of 224 genes (5.5%), including genes in all major functional categories, were found to be partially or completely deleted. Deletions are not distributed randomly throughout the genome but instead tend to be aggregated. The distinct deletions in some aggregations appear in closely related isolates, suggesting a genomically disruptive process specific to an individual mycobacterial lineage. Other genomic

SUBMITTER: Tsolaki AG 

PROVIDER: S-EPMC387340 | biostudies-literature | 2004 Apr

REPOSITORIES: biostudies-literature