Serine 216 phosphorylation of estrogen receptor α in neutrophils: migration and infiltration into the mouse uterus.

ABSTRACT:

Background

Whereas estrogen receptors are present in immune cells, it is not known if they are phosphorylated to regulate immune cell functions. Here we determined the phosphorylation status of estrogen receptor α (ERα) at residue serine 216 in mouse neutrophils and examined its role in migration and infiltration. Serine 216 is the conserved phosphorylation site within the DNA binding domains found in the majority of nuclear receptors.

Methodology/principal findings

A phospho-peptide antibody specific to phosphorylated serine 216 and ERα KO mice were utilized in immunohistochemistry, double immuno-staining or Western blot to detect phosphorylation of ERα in peripheral blood as well as infiltrating neutrophils in the mouse uterus. Transwell assays were performed to examine mi

SUBMITTER: Shindo S 

PROVIDER: S-EPMC3873424 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature