Systematic microRNA analysis identifies ATP6V0C as an essential host factor for human cytomegalovirus replication.

ABSTRACT: Recent advances in microRNA target identification have greatly increased the number of putative targets of viral microRNAs. However, it is still unclear whether all targets identified are biologically relevant. Here, we use a combined approach of RISC immunoprecipitation and focused siRNA screening to identify targets of HCMV encoded human cytomegalovirus that play an important role in the biology of the virus. Using both a laboratory and clinical strain of human cytomegalovirus, we identify over 200 putative targets of human cytomegalovirus microRNAs following infection of fibroblast cells. By comparing RISC-IP profiles of miRNA knockout viruses, we have resolved specific interactions between human cytomegalovirus miRNAs and the top candidate target transcripts and validated regulation by

SUBMITTER: Pavelin J 

PROVIDER: S-EPMC3873435 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature