INTER- AND INTRASPECIFIC COMMUNITY STRUCTURE WITHIN THE DIATOM GENUS <i>PSEUDO-NITZSCHIA</i> (BACILLARIOPHYCEAE).

ABSTRACT: Pseudo-nitzschia-specific PCR primers (PnAll F/R) were designed to amplify a polymorphic region of the internal transcribed spacer 1 (ITS1) from at least 11 Pseudo-nitzschia species. The primers were used to generate environmental clone libraries from Puget Sound, Washington, and Vancouver Island, British Columbia, to confirm that the primers were specific for Pseudo-nitzschia and to determine the extent of ITS1 sequence diversity within individual species. All environmental ITS1 sequences generated with PnAll primers displayed the greatest similarity to known Pseudo-nitzschia ITS1 sequences. The length of cloned ITS1 fragments differed among species but was conserved within a species. Intraspecific genotypes exhibited <3% sequence divergence for seven of the 10

SUBMITTER: Hubbard KA 

PROVIDER: S-EPMC3873754 | biostudies-literature | 2008 Jun

REPOSITORIES: biostudies-literature