Imaging of RNAs in live cells with spectrally diverse small molecule fluorophores.

ABSTRACT: We have developed new protein-based tags for use in imaging of RNAs in living cells. These tags are based on the MS2 phage coat protein fused to either E. coli dihydrofolate reductase or the SNAP tag. Labeled RNAs can be visualized with fluorophores spanning the visible spectra range.

SUBMITTER: Carrocci TJ 

PROVIDER: S-EPMC3873762 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature