Using bovine pituitary extract to increase proliferation of keratocytes and maintain their phenotype in vitro.

ABSTRACT:

Aim

To investigate the effects of bovine pituitary extract on the proliferation of keratocytes and maintaining the keratocyte phenotype in vitro.

Methods

Single keratocytes were isolated by enzyme digestion for in vitro culture. Three groups were designed according to the different culture media: a bovine pituitary extract (BPE) group, a fetal bovine serum (FBS) group and the control group. The phenotypes and proliferation of cultured cells were evaluated by morphology, immunofluorescent staining and mRNA expression of CD34, Lumican, VSX1, α-SMA and proliferating cell nuclear antigen (PCNA). In the BPE group, cells underwent serial subcultivation, and their phenotypes were identified by immunofluorescent staining. To analyze the proliferation of keratocytes in different conc

SUBMITTER: Xu ZZ 

PROVIDER: S-EPMC3874512 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature