Molecular analysis of ATP-sensitive K⁺ channel subunits expressed in mouse vas deferens myocytes.

ABSTRACT:

Background and purpose

ATP-sensitive K(+)(K(ATP)) channels, which are composed of K(IR)6.x associated with sulphonylurea receptor (SUR) subunits, have been detected in native smooth muscle cells, but it is currently not known which of these is expressed in mouse vas deferens myocytes.

Experimental approach

Pharmacological and electrophysiological properties of K(ATP) channels in mouse vas deferens myocytes were investigated using patch clamp techniques. Molecular biological analyses were performed to examine the properties of these K(ATP) channels.

Key results

During conventional whole-cell recording, pinacidil elicited an inward current that was suppressed by glibenclamide, a sulfonylurea agent, and by U-37883A, a selective K(IR)6.1 blocker. When 0.3 mM ATP was added

SUBMITTER: Iwasa K 

PROVIDER: S-EPMC3874703 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature