Rapid identification of sequences for orphan enzymes to power accurate protein annotation.

ABSTRACT: The power of genome sequencing depends on the ability to understand what those genes and their proteins products actually do. The automated methods used to assign functions to putative proteins in newly sequenced organisms are limited by the size of our library of proteins with both known function and sequence. Unfortunately this library grows slowly, lagging well behind the rapid increase in novel protein sequences produced by modern genome sequencing methods. One potential source for rapidly expanding this functional library is the "back catalog" of enzymology--"orphan enzymes," those enzymes that have been characterized and yet lack any associated sequence. There are hundreds of orphan enzymes in the Enzyme Commission (EC) database alone. In this study, we demonstrate how this orphan en

SUBMITTER: Ramkissoon KR 

PROVIDER: S-EPMC3875567 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature