Orthogonal analytical methods for botanical standardization: determination of green tea catechins by qNMR and LC-MS/MS.

ABSTRACT: The development of analytical methods for parallel characterization of multiple phytoconstituents is essential to advance the quality control of herbal products. While chemical standardization is commonly carried out by targeted analysis using gas or liquid chromatography-based methods, more universal approaches based on quantitative (1)H NMR (qHNMR) measurements are being used increasingly in the multi-targeted assessment of these complex mixtures. The present study describes the development of a 1D qHNMR-based method for simultaneous identification and quantification of green tea constituents. This approach utilizes computer-assisted (1)H iterative Full Spin Analysis (HiFSA) and enables rapid profiling of seven catechins in commercial green tea extracts. The qHNMR results were cross-vali

SUBMITTER: Napolitano JG 

PROVIDER: S-EPMC3875626 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature