Identification of novel tumor suppressor proteases by degradome profiling of colorectal carcinomas.

ABSTRACT: Proteolytic enzymes play important roles during tumor development and progression through their ability to promote cell growth or by facilitating the invasion of surrounding tissues. The human genome contains more than 570 protease-coding genes, many of them forming functional networks, which has forced the use of global strategies for the analysis of this group of enzymes. In this study, we have designed a new quantitative PCR-based device for profiling the entire degradome in human malignancies. We have used this method to evaluate protease expression levels in colorectal carcinomas with the finding that most proteases with altered expression in these tumors exert their function in the extracellular compartment. In addition, we have found that among genes encoding repressed proteases the

SUBMITTER: Fraile JM 

PROVIDER: S-EPMC3875759 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature