Evaluation of the mtDNA-COII Region Based Species Specific Assay for Identifying Members of the Anopheles culicifacies Species Complex.

ABSTRACT:

Background

Anopheles culicifacies, a major malarial vector has been recognized as a complex of five sibling species, A, B, C, D and E. These sibling species exhibit varied vectorial capacity, host specificity and susceptibility to malarial parasites/ insecticides. In this study, a PCR assay developed earlier for distinguishing the five individual species was validated on samples of An. culicifacies collected from various parts of India.

Methods

The samples were initially screened using the rDNA-ITS2 region based primers which categorised the samples into either A/D group or B/C/E group. A proportion of samples belonging to each group were subjected to the mtDNA-COII PCR assay for identifying individual species.

Results

Among the 615 samples analysed by rDNA-ITS2 PCR a

SUBMITTER: Manonmani AM 

PROVIDER: S-EPMC3875882 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature