Lipid bilayer nanodisc platform for investigating polyprenol-dependent enzyme interactions and activities.

ABSTRACT: Membrane-bound polyprenol-dependent pathways are important for the assembly of essential glycoconjugates in all domains of life. However, despite their prevalence, the functional significance of the extended linear polyprenyl groups in the interactions of the glycan substrates, the biosynthetic enzymes that act upon them, and the membrane bilayer in which they are embedded remains a mystery. These interactions are investigated simultaneously and uniquely through application of the nanodisc membrane technology. The Campylobacter jejuni N-linked glycosylation pathway has been chosen as a model pathway in which all of the enzymes and substrates are biochemically accessible. We present the functional reconstitution of two enzymes responsible for the early membrane-committed steps in glycan ass

SUBMITTER: Hartley MD 

PROVIDER: S-EPMC3876266 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature