Error analysis of deep sequencing of phage libraries: peptides censored in sequencing.

ABSTRACT: Next-generation sequencing techniques empower selection of ligands from phage-display libraries because they can detect low abundant clones and quantify changes in the copy numbers of clones without excessive selection rounds. Identification of errors in deep sequencing data is the most critical step in this process because these techniques have error rates >1%. Mechanisms that yield errors in Illumina and other techniques have been proposed, but no reports to date describe error analysis in phage libraries. Our paper focuses on error analysis of 7-mer peptide libraries sequenced by Illumina method. Low theoretical complexity of this phage library, as compared to complexity of long genetic reads and genomes, allowed us to describe this library using convenient linear vector and operator fr

SUBMITTER: Matochko WL 

PROVIDER: S-EPMC3876701 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature