Estimating extracellular spike waveforms from CA1 pyramidal cells with multichannel electrodes.

ABSTRACT: Extracellular (EC) recordings of action potentials from the intact brain are embedded in background voltage fluctuations known as the "local field potential" (LFP). In order to use EC spike recordings for studying biophysical properties of neurons, the spike waveforms must be separated from the LFP. Linear low-pass and high-pass filters are usually insufficient to separate spike waveforms from LFP, because they have overlapping frequency bands. Broad-band recordings of LFP and spikes were obtained with a 16-channel laminar electrode array (silicone probe). We developed an algorithm whereby local LFP signals from spike-containing channel were modeled using locally weighted polynomial regression analysis of adjoining channels without spikes. The modeled LFP signal was subtracted from the rec

SUBMITTER: Molden S 

PROVIDER: S-EPMC3876992 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature