Using standard optical flow cytometry for synchronizing proliferating cells in the G1 phase.

ABSTRACT: Cell cycle research greatly relies on synchronization of proliferating cells. However, effective synchronization of mammalian cells is commonly achieved by long exposure to one or more cell cycle blocking agents. These chemicals are, by definition, hazardous (some more than others), pose uneven cell cycle arrest, thus introducing unwanted variables. The challenge of synchronizing proliferating cells in G1 is even greater; this process typically involves the release of drug-arrested cells into the cycle that follows, a heterogeneous process that can truly limit synchronization. Moreover, drug-based synchronization decouples the cell cycle from cell growth in ways that are understudied and intolerable for those who investigate the relationship between these two processes. In this study we sh

SUBMITTER: Vecsler M 

PROVIDER: S-EPMC3877101 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature