Extensive adipogenic and osteogenic differentiation of patterned human mesenchymal stem cells in a microfluidic device.

ABSTRACT: Microtechnology offers great prospects for cellular research by enabling controlled experimental conditions that cannot be achieved by traditional methods. This study demonstrates the use of a microfluidic platform for long-term cultivation (3 weeks) of human mesenchymal stem-like cells (MSCs), a cell population of high interest for tissue engineering. The typical high motility of the MSCs required a strategy for preventing cells from inhabiting the feeding channels and thus interfere with a steady perfusion of medium to the cell cultivation chamber. Hence, a straightforward and long-term patterning method was developed and implemented for reliable cell positioning within the device. This method was based on the modification of a polystyrene substrate into cell supportive and non-supportiv

SUBMITTER: Tenstad E 

PROVIDER: S-EPMC3877732 | biostudies-literature | 2010 Mar

REPOSITORIES: biostudies-literature