Quantifying RNA allelic ratios by microfluidic multiplex PCR and sequencing.

ABSTRACT: We developed a targeted RNA sequencing method that couples microfluidics-based multiplex PCR and deep sequencing (mmPCR-seq) to uniformly and simultaneously amplify up to 960 loci in 48 samples independently of their gene expression levels and to accurately and cost-effectively measure allelic ratios even for low-quantity or low-quality RNA samples. We applied mmPCR-seq to RNA editing and allele-specific expression studies. mmPCR-seq complements RNA-seq for studying allelic variations in the transcriptome.

SUBMITTER: Zhang R 

PROVIDER: S-EPMC3877737 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature