Endoplasmic reticulum stress mediates house dust mite-induced airway epithelial apoptosis and fibrosis.

ABSTRACT:

Background

The endoplasmic reticulum (ER) stress response participates in many chronic inflammatory and autoimmune diseases. In the current study, we sought to examine the contribution of ER stress transducers in the pathogenesis of three principal facets of allergic asthma: inflammation, airway fibrosis, and airways hyperresponsiveness.

Methods

House Dust Mite (HDM) was used as an allergen for in vitro and in vivo challenge of primary human and murine airway epithelial cells. ER stress transducers were modulated using specific small interfering RNAs (siRNAs) in vivo. Inflammation, airway remodeling, and hyperresponsiveness were measured by total bronchoalveolar lavage (BAL) cell counts, determination of collagen, and methacholine responsiveness in mice, respectively.

Res

SUBMITTER: Hoffman SM 

PROVIDER: S-EPMC3877992 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature