Extracellular cholesterol oxidase from Rhodococcus sp.: isolation and molecular characterization.

ABSTRACT:

Background

Cholesterol oxidase (CHO) has various clinical and industrial applications. Recently, microbial CHO have received a great attention for their wide usage in medicine. Here, taxonomic characterizations of isolated strain from soil, optimization of the conditions for CHO production and biochemical characterizations of produced CHO enzyme were described. Finally, CHO gene was cloned into a cloning vector.

Methods

Various samples were collected and cultivated in a screening medium consisting of cholesterol. For isolation of CHO-producing bacteria, well-grown colonies were inoculated into an optimized medium. Different biochemical and microbiological tests were performed on isolated bacteria to identify their properties. For phylogenic analysis, a partial sequence of l6

SUBMITTER: Lashkarian H 

PROVIDER: S-EPMC3878146 | biostudies-literature | 2010 Jan-Apr

REPOSITORIES: biostudies-literature