Differential transcriptome analysis of glandular and filamentous trichomes in Artemisia annua.

ABSTRACT:

Background

The medicinal plant Artemisia annua is covered with filamentous trichomes and glandular, artemisinin producing trichomes. A high artemisinin supply is needed at a reduced cost for treating malaria. Artemisinin production in bioreactors can be facilitated if a better insight is obtained in the biosynthesis of artemisinin and other metabolites. Therefore, metabolic activities of glandular and filamentous trichomes were investigated at the transcriptome level.

Results

By laser pressure catapulting, glandular and filamentous trichomes as well as apical and sub-apical cells from glandular trichomes were collected and their transcriptome was sequenced using Illumina RNA-Seq. A de novo transcriptome was assembled (Trinity) and studied with a differential expression analy

SUBMITTER: Soetaert SS 

PROVIDER: S-EPMC3878173 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature