Optimized heterologous transfection of viable adult organotypic brain slices using an enhanced gene gun.

ABSTRACT:

Background

Organotypic brain slices (OTBS) are an excellent experimental compromise between the facility of working with cell cultures and the biological relevance of using animal models where anatomical, morphological, and cellular function of specific brain regions can be maintained. The biological characteristics of OTBS can subsequently be examined under well-defined conditions. They do, however, have a number of limitations; most brain slices are derived from neonatal animals, as it is difficult to properly prepare and maintain adult OTBS. There are ample problems with tissue integrity as OTBS are delicate and frequently become damaged during the preparative stages. Notwithstanding these obstacles, the introduced exogenous proteins into both neuronal cells, and cells imbedded

SUBMITTER: Arsenault J 

PROVIDER: S-EPMC3878247 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature