A BAC based physical map and genome survey of the rice false smut fungus Villosiclava virens.

ABSTRACT:

Background

Rice false smut caused by Villosiclava virens is a devastating fungal disease that spreads in major rice-growing regions throughout the world. However, the genomic information for this fungal pathogen is limited and the pathogenic mechanism of this disease is still not clear. To facilitate genetic, molecular and genomic studies of this fungal pathogen, we constructed the first BAC-based physical map and performed the first genome survey for this species.

Results

High molecular weight genomic DNA was isolated from young mycelia of the Villosiclava virens strain UV-8b and a high-quality, large-insert and deep-coverage Bacterial Artificial Chromosome (BAC) library was constructed with the restriction enzyme HindIII. The BAC library consisted of 5,760 clones, which co

SUBMITTER: Wang X 

PROVIDER: S-EPMC3878662 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature