A phylogenomic approach to bacterial subspecies classification: proof of concept in Mycobacterium abscessus.

ABSTRACT:

Background

Mycobacterium abscessus is a rapidly growing mycobacterium that is often associated with human infections. The taxonomy of this species has undergone several revisions and is still being debated. In this study, we sequenced the genomes of 12 M. abscessus strains and used phylogenomic analysis to perform subspecies classification.

Results

A data mining approach was used to rank and select informative genes based on the relative entropy metric for the construction of a phylogenetic tree. The resulting tree topology was similar to that generated using the concatenation of five classical housekeeping genes: rpoB, hsp65, secA, recA and sodA. Additional support for the reliability of the subspecies classification came from the analysis of erm41 and ITS gene sequences, s

SUBMITTER: Tan JL 

PROVIDER: S-EPMC3878664 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature