Correlation of WWOX, RUNX2 and VEGFA protein expression in human osteosarcoma.

ABSTRACT:

Background

To investigate associations between WW domain-containing oxidoreductase (WWOX), runt-related transcription factor 2 (RUNX2) and vascular endothelial growth factor alpha (VEGFA) in human osteosarcoma (OS).

Methods

Copy number aberrations of WWOX, RUNX2and VEGFA genes were detected by microarray comparative genomic hybridization (aCGH) in 10 fresh OS tissue samples. VEGFA gene alterations were also investigated and validated by fluorescence in situ hybridization (FISH) in 54 formalin-fixed and paraffin-embedded (FFPE) OS samples. Protein expression of WWOX, RUNX2 and VEGFA were examined in 54 FFPE OS samples by immunohistochemistry (IHC).

Results

Analysis of previously published OS aCGH data (GSE9654) and aCGH data from this study (GSE19180) identified signif

SUBMITTER: Yang J 

PROVIDER: S-EPMC3878685 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature