Plasma and urinary amino acid metabolomic profiling in patients with different levels of kidney function.

ABSTRACT:

Background and objectives

Patients with CKD display altered plasma amino acid profiles. This study estimated the association between the estimated GFR and urinary and plasma amino acid profiles in CKD patients.

Design, setting, participants, & measurements

Urine and plasma samples were taken from 52 patients with different stages of CKD, and plasma samples only were taken from 25 patients on maintenance hemodialysis. Metabolic profiling was performed by liquid chromatography coupled with tandem mass spectrometry after phenylisothiocyanate derivatization.

Results

Most plasma amino acid concentrations were decreased in hemodialysis patients, whereas proline, citrulline, asparagine, asymmetric dimethylarginine, and hydroxykynurenine levels were increased (P<0.05). Both p

SUBMITTER: Duranton F 

PROVIDER: S-EPMC3878706 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature