Microarray analysis provides new insights into the function of apolipoprotein O in HepG2 cell line.

ABSTRACT:

Background

Apolipoprotein O (apoO) is a new member of the apolipoprotein family. However, data on its physiological functions are limited and inconsistent. Using a microarray expression analysis, this study explored the function of apoO in liver cells.

Methods

HepG2 cells were treated either with oleic acid or tumor necrosis factor-α for 24 h. mRNA and protein expression of apoO were assessed by quantitative real-time PCR (qRT-PCR) and Western blot respectively. An efficient lentiviral siRNA vector targeting the human apoO gene was designed and constructed. The gene expression profile of HepG2 human hepatocellular carcinoma cells transfected with the apoO silencing vector was investigated using a whole-genome oligonucleotide microarray. The expression levels of some altered

SUBMITTER: Wu CL 

PROVIDER: S-EPMC3878747 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature