Phosphoproteomics and bioinformatics analyses of spinal cord proteins in rats with morphine tolerance.

ABSTRACT:

Introduction

Morphine is the most effective pain-relieving drug, but it can cause unwanted side effects. Direct neuraxial administration of morphine to spinal cord not only can provide effective, reliable pain relief but also can prevent the development of supraspinal side effects. However, repeated neuraxial administration of morphine may still lead to morphine tolerance.

Methods

To better understand the mechanism that causes morphine tolerance, we induced tolerance in rats at the spinal cord level by giving them twice-daily injections of morphine (20 µg/10 µL) for 4 days. We confirmed tolerance by measuring paw withdrawal latencies and maximal possible analgesic effect of morphine on day 5. We then carried out phosphoproteomic analysis to investigate the global phosphoryla

SUBMITTER: Liaw WJ 

PROVIDER: S-EPMC3879267 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature