Elucidating novel hepatitis C virus-host interactions using combined mass spectrometry and functional genomics approaches.

ABSTRACT: More than 170 million people worldwide are infected with the hepatitis C virus (HCV), for which future therapies are expected to rely upon a combination of oral antivirals. For a rapidly evolving virus like HCV, host-targeting antivirals are an attractive option. To decipher the role of novel HCV-host interactions, we used a proteomics approach combining immunoprecipitation of viral-host protein complexes coupled to mass spectrometry identification and functional genomics RNA interference screening of HCV partners. Here, we report the proteomics analyses of protein complexes associated with Core, NS2, NS3/4A, NS4B, NS5A, and NS5B proteins. We identified a stringent set of 98 human proteins interacting specifically with one of the viral proteins. The overlap with previous virus-host interac

SUBMITTER: Germain MA 

PROVIDER: S-EPMC3879614 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature