Proteome informatics research group (iPRG)_2012: a study on detecting modified peptides in a complex mixture.

ABSTRACT: The proteome informatics research group of the Association of Biomolecular Resource Facilities conducted a study to assess the community's ability to detect and characterize peptides bearing a range of biologically occurring post-translational modifications when present in a complex peptide background. A data set derived from a mixture of synthetic peptides with biologically occurring modifications combined with a yeast whole cell lysate as background was distributed to a large group of researchers and their results were collectively analyzed. The results from the twenty-four participants, who represented a broad spectrum of experience levels with this type of data analysis, produced several important observations. First, there is significantly more variability in the ability to assess whe

SUBMITTER: Chalkley RJ 

PROVIDER: S-EPMC3879627 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature