The nucleosome regulates the usage of polyadenylation sites in the human genome.

ABSTRACT:

Background

It has been reported that 3' end processing is coupled to transcription and nucleosome depletion near the polyadenylation sites in many species. However, the association between nucleosome occupancy and polyadenylation site usage is still unclear.

Results

By systematic analysis of high-throughput sequencing datasets from the human genome, we found that nucleosome occupancy patterns are different around the polyadenylation sites, and that the patterns associate with both transcription termination and recognition of polyadenylation sites. Upstream of proximal polyadenylation sites, RNA polymerase II accumulated and nucleosomes were better positioned compared with downstream of the sites. Highly used proximal polyadenylation sites had higher upstream nucleosome level

SUBMITTER: Huang H 

PROVIDER: S-EPMC3879661 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature