Transcriptomes of the major human pancreatic cell types.

ABSTRACT:

Aims/hypothesis

We sought to determine the mRNA transcriptome of all major human pancreatic endocrine and exocrine cell subtypes, including human alpha, beta, duct and acinar cells. In addition, we identified the cell type-specific distribution of transcription factors, signalling ligands and their receptors.

Methods

Islet samples from healthy human donors were enzymatically dispersed to single cells and labelled with cell type-specific surface-reactive antibodies. Live endocrine and exocrine cell subpopulations were isolated by FACS and gene expression analyses were performed using microarray analysis and quantitative RT-PCR. Computational tools were used to evaluate receptor-ligand representation in these populations.

Results

Analysis of the transcriptomes of alpha,

SUBMITTER: Dorrell C 

PROVIDER: S-EPMC3880150 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature