Macrophages mediate a switch between canonical and non-canonical Wnt pathways in canine mammary tumors.

ABSTRACT:

Objective

According to the current hypothesis, tumor-associated macrophages (TAMs) are "corrupted" by cancer cells and subsequently facilitate, rather than inhibit, tumor metastasis. Because the molecular mechanisms of cancer cell-TAM interactions are complicated and controversial we aimed to better define this phenomenon.

Methods and results

Using microRNA microarrays, Real-time qPCR and Western blot we showed that co-culture of canine mammary tumor cells with TAMs or treatment with macrophage-conditioned medium inhibited the canonical Wnt pathway and activated the non-canonical Wnt pathway in tumor cells. We also showed that co-culture of TAMs with tumor cells increased expression of canonical Wnt inhibitors in TAMs. Subsequently, we demonstrated macrophage-induced invasiv

SUBMITTER: Krol M 

PROVIDER: S-EPMC3880277 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature