Development of a multiplexed bead-based suspension array for the detection and discrimination of pospiviroid plant pathogens.

ABSTRACT: Efficient and reliable diagnostic tools for the routine indexing and certification of clean propagating material are essential for the management of pospiviroid diseases in horticultural crops. This study describes the development of a true multiplexed diagnostic method for the detection and identification of all nine currently recognized pospiviroid species in one assay using Luminex bead-based suspension array technology. In addition, a new data-driven, statistical method is presented for establishing thresholds for positivity for individual assays within multiplexed arrays. When applied to the multiplexed array data generated in this study, the new method was shown to have better control of false positives and false negative results than two other commonly used approaches for setting th

SUBMITTER: van Brunschot SL 

PROVIDER: S-EPMC3880322 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature