Unknown

Dataset Information

0

Multiplexed MS/MS for improved data-independent acquisition.

ABSTRACT: In mass spectrometry-based proteomics, data-independent acquisition (DIA) strategies can acquire a single data set useful for both identification and quantification of detectable peptides in a complex mixture. However, DIA data are noisy owing to a typical five- to tenfold reduction in precursor selectivity compared to data obtained with data-dependent acquisition or selected reaction monitoring. We demonstrate a multiplexing strategy, MSX, for DIA analysis that increases precursor selectivity fivefold.

SUBMITTER: Egertson JD 

PROVIDER: S-EPMC3881977 | biostudies-literature | 2013 Aug

REPOSITORIES: biostudies-literature

Json Xml
altmetric image

Publications

In mass spectrometry-based proteomics, data-independent acquisition (DIA) strategies can acquire a single data set useful for both identification and quantification of detectable peptides in a complex mixture. However, DIA data are noisy owing to a typical five- to tenfold reduction in precursor selectivity compared to data obtained with data-dependent acquisition or selected reaction monitoring. We demonstrate a multiplexing strategy, MSX, for DIA analysis that increases precursor selectivity f  ...[more]

Similar Datasets

Unknown Multiplexed quantification for data-independent acquisition.
| S-EPMC4472008 | biostudies-literature
Unknown Multiplexed peptide analysis using data-independent acquisition and Skyline.
| S-EPMC5127711 | biostudies-literature
Unknown Ranking Fragment Ions Based on Outlier Detection for Improved Label-Free Quantification in Data-Independent Acquisition LC-MS/MS.
| S-EPMC4739871 | biostudies-literature
Unknown Mass Defect-Based DiLeu Tagging for Multiplexed Data-Independent Acquisition.
| S-EPMC7438256 | biostudies-literature
Unknown Comparative Proteome Signatures of Trace Samples by Multiplexed Data-Independent Acquisition.
| S-EPMC8717550 | biostudies-literature
Unknown Data-independent acquisition-based SWATH-MS for quantitative proteomics: a tutorial.
| S-EPMC6088389 | biostudies-literature
Unknown The Isotopic Ac-IP Tag Enables Multiplexed Proteome Quantification in Data-Independent Acquisition Mode.
| S-EPMC8209779 | biostudies-literature
Unknown Sensitive Immunopeptidomics by Leveraging Available Large-Scale Multi-HLA Spectral Libraries, Data-Independent Acquisition, and MS/MS Prediction.
| S-EPMC8724634 | biostudies-literature
Unknown Improved SILAC Quantification with Data-Independent Acquisition to Investigate Bortezomib-Induced Protein Degradation.
| S-EPMC8256668 | biostudies-literature
Unknown Data on protein changes of chick vitreous during normal eye growth using data-independent acquisition (SWATH-MS).
| S-EPMC7195514 | biostudies-literature