Analysis of gene expression in wild-type and Notch1 mutant retinal cells by single cell profiling.

ABSTRACT:

Background

The vertebrate retina comprises sensory neurons, the photoreceptors, as well as many other types of neurons and one type of glial cell. These cells are generated by multipotent and restricted retinal progenitor cells (RPCs), which express Notch1. Loss of Notch1 in RPCs late during retinal development results in the overproduction of rod photoreceptors at the expense of interneurons and glia.

Results

To examine the molecular underpinnings of this observation, microarray analysis of single retinal cells from wild-type or Notch1 conditional knockout retinas was performed. In situ hybridization was carried out to validate some of the findings.

Conclusions

The majority of Notch1-mutant cells lost expression of known Notch target genes. These cells also had low l

SUBMITTER: Mizeracka K 

PROVIDER: S-EPMC3882027 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature