Efficient detection of hydrogen bonds in dynamic regions of RNA by sensitivity-optimized NMR pulse sequences.

ABSTRACT: Improved Sensitivity: Efficient NMR experiments are presented for determining the secondary structure in large and dynamic RNAs using J-couplings across hydrogen bonds. The experiments provide up to eight-fold improved sensitivity and thus enable detection of base pairs in dynamic regions even in large RNAs.

SUBMITTER: Dallmann A 

PROVIDER: S-EPMC3882076 | biostudies-literature | 2013 Sep

REPOSITORIES: biostudies-literature