Exploring the pH-dependent substrate transport mechanism of FocA using molecular dynamics simulation.

ABSTRACT: FocA belongs to the formate-nitrate transporter family and plays an essential role in the export and uptake of formate in organisms. According to the available crystal structures, the N-terminal residues of FocA are structurally featureless at physiological conditions but at reduced pH form helices to harbor the cytoplasmic entrance of the substrate permeation pathway, which apparently explains the cessation of electrical signal observed in electrophysiological experiments. In this work, we found by structural analysis and molecular dynamics simulations that those N-terminal helices cannot effectively preclude the substrate permeation. Equilibrium simulations and thermodynamic calculations suggest that FocA is permeable to both formate and formic acid, the latter of which is transparent to

SUBMITTER: Lv X 

PROVIDER: S-EPMC3882453 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature