An explicit formulation approach for the analysis of calcium binding to EF-hand proteins using isothermal titration calorimetry.

ABSTRACT: We present an improved and extended version of a recently proposed mathematical approach for modeling isotherms of ligand-to-macromolecule binding from isothermal titration calorimetry. Our approach uses ordinary differential equations, solved implicitly and numerically as initial value problems, to provide a quantitative description of the fraction bound of each competing member of a complex mixture of macromolecules from the basis of general binding polynomials. This approach greatly simplifies the formulation of complex binding models. In addition to our generalized, model-free approach, we have introduced a mathematical treatment for the case where ligand is present before the onset of the titration, essential for data analysis when complete removal of the binding partner may disrupt t

SUBMITTER: Keeler C 

PROVIDER: S-EPMC3882476 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature