Genome-wide analysis of the rat colon reveals proximal-distal differences in histone modifications and proto-oncogene expression.

ABSTRACT: Since disease susceptibility of the intestine exhibits an anatomical bias, we propose that the chromatin landscape, especially the site-specific epigenetic differences in histone modification patterns throughout the colonic longitudinal axis, contributes to the differential incidence of site-specific pathology. To test this hypothesis, we assessed the chromatin structure associated with gene expression profiles in the rat proximal and distal colon by globally correlating chromatin immunoprecipitation next-generation sequencing analysis (ChIP-Seq) with mRNA transcription (RNA-Seq) data. Crypts were isolated from the proximal and distal colonic regions from rats maintained on a semipurified diet, and mRNA gene expression profiles were generated by RNA-Seq. The remaining isolated crypts were

SUBMITTER: Triff K 

PROVIDER: S-EPMC3882682 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature