Human ovarian tissue vitrification/warming has minor effect on the expression of apoptosis-related genes.

ABSTRACT:

Background

In this study, we evaluated the incidence of apoptosis at the ultrastructural levels and expression of some apoptosis-related genes in vitrified human ovarian tissue just after warming.

Methods

Human ovarian tissue biopsies from 23 women after caesarean section were transported to the laboratory within 2 hours, and then they were cut into small pieces. Some pieces were vitrified and warmed and the other samples were considered as control. Apoptosis was assessed by a transmission electron microscope and also by molecular analysis of pro-apoptotic (Fas, FasL, Bax, p53, caspase8, and caspase3) and antiapoptotic (Bcl-2 and BIRC5) genem RNA levels using real-time RT-PCR before and after vitrification.

Results

No sign of apoptosis was shown ultrastructurally in v

SUBMITTER: Abdollahi M 

PROVIDER: S-EPMC3882920 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature