Cytotoxicity and apoptosis induced by nanobacteria in human breast cancer cells.

ABSTRACT:

Background

The existing evidence that nanobacteria (NB) are closely associated with human disease is overwhelming. However, their potential toxicity against cancer cells has not yet been reported. The objective of this study was to investigate the cytotoxic effects of NB and nanohydroxyapatites (nHAPs) against human breast cancer cells and to elucidate the mechanisms of action underlying their cytotoxicity.

Methodology/principal findings

NB were isolated from calcified placental tissue, and nHAPs were artificially synthesized. The viability of the MDA-MB-231 human breast cancer cell line was tested by using the Kit-8 cell counting kit assay. Apoptosis was examined by transmission electron microscopy and flow cytometry. The endocytosis of NB and nHAPs by MDA-MB-231 cells was

SUBMITTER: Zhang MJ 

PROVIDER: S-EPMC3883551 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature