In-depth proteomic and glycomic analysis of the adult-stage Cooperia oncophora excretome/secretome.

ABSTRACT: Cooperia oncophora is one of the most common intestinal parasitic nematodes in cattle worldwide. To date, C. oncophora infections are treated using broad-spectrum anthelmintics. However, during the past decade, reports of anthelmintic resistance in this parasite species have emerged worldwide, necessitating new avenues for its control, possibly through vaccination. In this frame, we analyzed the adult-stage C. oncophora excretome/secretome (ES), covering both the protein and glycan components, since this fraction constitutes the primary interface between parasite and host and may hold potential vaccine candidates. Two-dimensional gel electrophoretic separation of the ES material enabled the MALDI-TOF mass spectrometry (MS)-directed identification of 12 distinct proteins, grouped in three s

SUBMITTER: Borloo J 

PROVIDER: S-EPMC3883574 | biostudies-literature | 2013 Sep

REPOSITORIES: biostudies-literature