Assessment of whole genome amplification for sequence capture and massively parallel sequencing.

ABSTRACT: Exome sequence capture and massively parallel sequencing can be combined to achieve inexpensive and rapid global analyses of the functional sections of the genome. The difficulties of working with relatively small quantities of genetic material, as may be necessary when sharing tumor biopsies between collaborators for instance, can be overcome using whole genome amplification. However, the potential drawbacks of using a whole genome amplification technology based on random primers in combination with sequence capture followed by massively parallel sequencing have not yet been examined in detail, especially in the context of mutation discovery in tumor material. In this work, we compare mutations detected in sequence data for unamplified DNA, whole genome amplified DNA, and RNA originating

SUBMITTER: Hasmats J 

PROVIDER: S-EPMC3883664 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature