Probing the subcellular localization of hopanoid lipids in bacteria using NanoSIMS.

ABSTRACT: The organization of lipids within biological membranes is poorly understood. Some studies have suggested lipids group into microdomains within cells, but the evidence remains controversial due to non-native imaging techniques. A recently developed NanoSIMS technique indicated that sphingolipids group into microdomains within membranes of human fibroblast cells. We extended this NanoSIMS approach to study the localization of hopanoid lipids in bacterial cells by developing a stable isotope labeling method to directly detect subcellular localization of specific lipids in bacteria with ca. 60 nm resolution. Because of the relatively small size of bacterial cells and the relative abundance of hopanoid lipids in membranes, we employed a primary (2)H-label to maximize our limit of detection. Thi

SUBMITTER: Doughty DM 

PROVIDER: S-EPMC3883690 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature