Genome-scale analysis of DNA methylation in colorectal cancer using Infinium HumanMethylation450 BeadChips.

ABSTRACT: Illumina's Infinium HumanMethylation450 BeadChip arrays were used to examine genome-wide DNA methylation profiles in 22 sample pairs from colorectal cancer (CRC) and adjacent tissues and 19 colon tissue samples from cancer-free donors. We show that the methylation profiles of tumors and healthy tissue samples can be clearly distinguished from one another and that the main source of methylation variability is associated with disease status. We used different statistical approaches to evaluate the methylation data. In general, at the CpG-site level, we found that common CRC-specific methylation patterns consist of at least 15,667 CpG sites that were significantly different from either adjacent healthy tissue or tissue from cancer-free subjects. Of these sites, 10,342 were hypermethylated in

SUBMITTER: Naumov VA 

PROVIDER: S-EPMC3883769 | biostudies-literature | 2013 Sep

REPOSITORIES: biostudies-literature