Lipid in skeletal muscle myotubes is associated to the donors' insulin sensitivity and physical activity phenotypes.

ABSTRACT:

Objective

This study investigated the relationship between in vitro lipid content in myotubes and in vivo whole body phenotypes of the donors such as insulin sensitivity, intramyocellular lipids (IMCL), physical activity, and oxidative capacity.

Design and methods

Six physically active donors were compared to six sedentary lean and six T2DM. Lipid content was measured in tissues and myotubes by immunohistochemistry. Ceramides, triacylglycerols, and diacylglycerols (DAGs) were measured by LC-MS-MS and GC-FID. Insulin sensitivity was measured by hyperinsulinemic-euglycemic clamp (80 mU min⁻¹ m⁻²), maximal mitochondrial capacity (ATPmax) by ³¹P-MRS, physical fitness by VO₂max and physical activity level (PAL) by accelerometers.

Results

Myotubes cultured from physically a

SUBMITTER: Bajpeyi S 

PROVIDER: S-EPMC3883809 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature