PH gradient and distribution of streptococci, lactobacilli, prevotellae, and fusobacteria in carious dentine.

ABSTRACT:

Objectives

Caries process comprises acidogenic and aciduric bacteria that are responsible for lowering the pH and subsequent destruction of hydroxyapatite matrix in enamel and dentine. The aim of this study was to identify the correlation between the pH gradient of a carious lesion and proportion and distribution of four bacterial genera; lactobacilli, streptococci, prevotellae, and fusobacteria with regard to total load of bacteria.

Materials and methods

A total of 25 teeth with extensive dentinal caries were sampled in sequential layers. Using quantitative real-time PCR of 16S rRNA gene, we quantified the total load of bacteria as well as the proportion of the above-mentioned genera following pH measurement of each sample with a fine microelectrode.

Results

We demon

SUBMITTER: Kianoush N 

PROVIDER: S-EPMC3883984 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature